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Batch in-vitro digestibility assay for ruminants and monogastric animals

For the purpose of feed and food evaluation, in-vitro digestion and fermentation methods are ethically superior, faster and less expensive than in-vivo techniques. A good example of an in-vitro fermentation method is the in-vitro gas production technique, in which the gas evolved as a result of fermentation is used as the primary measurement. The method utilises the relationship between degradation and fermentative gas production to evaluate the nutritional parameters of the feed. Although the gas production technique is well-established in the area of ruminant feed evaluation, reports can also be found for nutritive evaluation of feed for monogastric animals and food evaluation for human. The benefits of in-vitro gas production techniques for digestibility evaluation include being able to run large batches simultaneously at a low cost, the ability to measure fermentation kinetics of soluble and insoluble fractions of feed or food, and the ability to easily make relative comparisons among different samples.

The Gas Endeavour® is our premier automatic instrument for continuous monitoring of fermentation gas (i.e., hydrogen, methane, carbon dioxide) released in in-vitro ruminant digestive models and in-vitro monogastric hindgut digestive model for monogastric animals and human. In comparison with the other in-vitro methods measuring the transformation of fermentable substrate, the Gas Endeavour allows highly accurate analysis for a large number of samples in a short time. In addition, the automated in-vitro protocol based on the Gas Endeavour significantly reduces the workload compared with manual analyses.

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Simplify the analysis of feed and food quality

Areas of application

related to in-vitro gas production analysis

Gas Endeavour is a powerful analytical tool to predict the rumen organic matter degradation through provision of kinetic information. Examples of application include estimating the energy content of feedstuffs for diet formulation, optimising efficiency of feed utilisation, ruminant output, assessing bioactive components with anti-methanogenic properties as well as anti-nutritive factors.

Like the rumen, the large intestine of simple-stomached animals is essentially a fermentation chamber where material is degraded by gut bacteria. The cumulative gas production technique can also be applied here and Gas Endeavour can be an ideal batch test platform of in-vitro hindgut digestion to investigate differences among feedstuffs, unweaned and adult animals and also the effect of enzymatic pre-treatment on fermentation kinetics.

The fermentation of dietary fibers by gut microbiota results in the generation of gas and production of short-chain fatty acids (SCFA), and can also provide selective substrates for growth of specific groups of bacteria that may enhance the intestinal health of the host. Gas Endeavour is an automated batch test platform of in-vitro lower gastrointestinal digestion and human fecal fermentation for studying microbiota population in lower gastrointestinal tract and fermentation properties of various dietary fibres.

Gas Endeavour® III

 – a novel tool for in-situ digestibility analysis

The Gas Endeavour III is a novel platform for analysing low gas volume and flow whenever there is a demand for high accuracy and precise measurements. The instrument can be used for research and industrial applications relating to animal nutrition, wastewater treatment, bioethanol fermentation, aerobic and anaerobic respiration, greenhouse gas emission, evaluation of microbial communities, and many more.

You can find more information on the Gas Endeavour page, the video, and the product brochure regarding in-vitro digestibility below. 

Benefits & advantages

Highly precise and accurate data

The Gas Endeavour gives you a better understanding of the digestion kinetics of fermentable substrates. The precision of measurement and data calculations have been validated with the highest quality and standards by scientists in international inter-laboratory studies.

Significant reduction in time consumption and labour requirements

Fully automated analytical procedures, extendable testing capacity and full control of experiments with remote access. The Gas Endeavour reduces the time consumption and labour requirement for in-vitro digestibility analysis and make the test being less skill dependent for precise and accurate data.

Standardised measurement procedures, data interpretations and reports

Real-time temperature and pressure compensations minimise the impact of possible variation in measurement conditions in analysis and standardise data presentation satisfying the highest demands for data accuracy and precision. An adaptive nonlinear mathematical model is also implemented in the latest released Gas Endeavour to achieve an outstanding high linearity for in-vitro gas production analysis in all measurement ranges.

Compact and modular design

The modular approach enables flexible system set-up, easy upgrading options and simple maintenance. The Gas Endeavour can easily be further expanded by connecting multiple instruments with a network switch in order to satisfy demands and shorten development times.

User-friendly operations with remote access

Simple to use and easy to learn. The web-based software application makes setting up and monitoring experiments very easy. The Gas Endeavour allows easy access from a remote location using any computer, smartphone or tablet.

References

Learn more about the Scientific References

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